Stable Cell Line Generation Service

Unlike the short term protein expression observed using transient transfection approaches generating cell lines using lentiviral vectors enables long term protein expression studies.

Stable cell line generation service. Creative biogene offers high quality fast turnaround and cost effective stable cell line generation service for protein expression gene knockdown or genome editing that meet your specific research needs. The transfection method determines the cell type for stable integration. Stable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected plasmid dna or reporter gene constructs into the genome. This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

All stable cell lines are produced according to individual client specifications with efficient gene delivery technology able to produce stable expression in clonal cell lines in 28 days service description. Point mutation at a defined locus are widely employed in functional studies drug discovery assays and recombinant protein production. Stable cell line generation cellomics technology llc provides high quality fast turnaround and cost effective stable cell line generation service for research biotechnology and pharmaceutical companies. Stable cell line generation stable cell line generation stable cell lines expressing a gene of interest or harboring knockout knockin or precise sequence changes e g.

Choosing to outsource this application may not only save time and effort but can also provide benefits from technical expertise and facilities dedicated to the generation of stable cell lines. Stable expression can be influenced by the transfection method used. Stable cell lines with specific gene over expression or knock down are very helpful in gene function analysis target discovery target vali. Major challenges for generation of stable cell lines are low transfection efficiency and or integration frequency.

Cell line development service options are available from several specialized providers.